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Literature Content === |
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 test method for American Ginseng Extract |
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Origin: USP |
Show: 287 |
Time: 2009-2-5 23:05:15 |
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Powdered American Ginseng Extract
»Powdered American Ginseng Extract is prepared from the pulverized dried roots of Panax quinquefoliusL.(Fam.Araliaceae),using suitable solvents,and dried to a powder.It contains not less than 10.0percent of total ginsenosides,calculated on the anhydrous basis.The ratio of starting crude plant material to Powdered Extract is between 3:1and 7:1.
Packaging and storage¡ª Preserve in tight,light-resistant containers.
Labeling¡ª The label states the Latin binomial and,following the official name,the part of the plant from which the article was derived.Label it to indicate the content of total ginsenosides,the extracting solvent used for preparation,and the ratio of the starting crude plant material to the Powdered Extract.It meets the labeling requirements under Botanical Extracts ¨¢565ñ.
Identification¡ª
B: The retention times of the peaks for ginsenosides Rg1,Re,Rb1,Rb2,Rc,and Rd in the chromatogram of the Test solutioncorrespond to those in the chromatogram of Standard solution 1,as obtained in the test for Content of ginsenosides.The ratio of the peak response for Rb2to the peak response for Rb1is less than 0.4,and the ratio of the peak response for Rg1to the peak response for Rb1is less than 0.3.The chromatogram of the Test solutionshows no significant peak at the retention time corresponding to that for ginsenoside Rf in the chromatogram of Standard solution 2,as obtained in the test for Content of ginsenosides.
Change to read: Microbial enumeration ¨¢2021ñ¡ª The total aerobic microbial count does not exceed 10 4cfu per g;the total combined molds and yeasts count does not exceed 1000cfu per g;and it meets the requirements of the tests for absence of Salmonellaspecies and Escherichia coli. USP28
Change to read: Content of ginsenosides¡ª
Solution A,Solution B,Mobile phase,Standard solution 1,Standard solution 2,and Chromatographic system¡ª Proceed as directed for Content of ginsenosidesunder American Ginseng.
Test solution¡ª Transfer a quantity USP28of Powdered Extract, equivalent to 5mg of ginsenosides, USP28accurately weighed,to a suitable container. Dissolve in 10.0mLof a mixture of water and alcohol (6:4)sonicating for 10minutes,and filter. USP28
Procedure¡ª Proceed as directed for Content of ginsenosidesunder American Ginseng.Calculate the percentages of individual ginsenosides in the portion of Powdered Extract taken by the formula:
1000( C/W)( rU/ rS), USP28 in which Cis the concentration,in mg per mL,of ginsenoside Rg 1,Re,Rb 1,Rc,Rb 2,or Rd in the appropriate Standard solution;Wis the weight,in mg,of Powdered Extract taken to prepare the Test solution;and rUand rSare the peak responses of ginsenoside Rg 1,Re,Rb 1,Rc,Rb 2,or Rd obtained from the Test solutionand the appropriate Standard solution,respectively.Calculate the percentage of total ginsenosides in the portion of Powdered Extract taken by adding the individual percentages |
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