Identification¡ª The retention times of the 11major peaks in the chromatogram of theTest solution correspond to those in the chromatogram of theStandard solution,as obtained in the test forContent of fatty acids.
The ratios of the concentration of lauric acid to the concentration of the respective fatty acid are in the following ranges.
Internal standard solution,Standard solution,and Chromatographic system¡ª Prepare as directed forContent of fatty acidsunderSaw Palmetto.
Test solution¡ª Transfer about 100mg of Extract,accurately weighed,to a pressure-proof,screw-capped vial,and add 3.0mLof a solution of sulfuric acid in methanol (5in 100).Heat at 100in an oil bath for 2hours,shaking from time to time.Allow to cool,and add 1.0mLofInternal standard solution,10.0mLof water,1g of sodium chloride,and 5mLof hexanes.Shake well,allow the layers to separate completely,and use the hexanes layer.[NOTE¡ªStore in a refrigerator until ready to use.]
Procedure¡ª Proceed as directed forContent of fatty acidsunderSaw Palmetto.Calculate the percentage of each fatty acid in the portion of Extract taken by the formula:
test method for Saw Palmetto Extract 
in an oil bath for 2hours,shaking from time to time.Allow to cool,and add 1.0mLof
